The purpose was to examine the combined effects of whey protein supplementation and high-volume resistance training (HVRT) on muscle mass and strength in healthy postmenopausal women. Postmenopausal women (n=12, age: 57 ± 4.3 years, weight: 72 ± 13.4 kg, height: 163 ± 5.7 cm, body mass index: 27 ± 5.4) consumed whey protein (40 grams) or placebo immediately following unilateral resistance training 2 days per week (Monday, Thursday) and consumed the opposite beverage after training the other side of the body on alternating days (Tuesday, Friday) for 10 weeks. For training, participants performed 3 sets at 30% baseline 1-repetition maximum (1RM) to volitional muscle fatigue for 4 exercises (leg curl, biceps curl, leg extension, triceps extension). Prior to and following training, assessments were made for upper and lower limb lean tissue mass (dual energy x-ray absorptiometry), muscle thickness of the elbow and knee flexors and extensors (ultrasound), and muscle strength (1RM leg curl, biceps curl, leg extension, triceps extension). There was a significant increase over time for muscle strength (biceps curl, leg extension, triceps extension; P=0.006) and muscle thickness (elbow flexors and extensors; P=0.022) with no differences between whey protein and placebo. High-volume resistance training improves indices of muscle mass and strength in postmenopausal women with no greater benefits observed from whey protein.