The study area lies within the Pine Lake Greenstone belt of the Glennie domain, 125 km northeast of LaRonge, SK. The Santoy shear zone hosts the Santoy 7, Santoy 8/8 East, and “Santoy Gap” gold deposits. This shear zone was studied using a structural and geochronological (ID-TIMS) approach in order to decipher its complex relationship with plutonism, deformation, alteration, and gold mineralization. Surface and subsurface mapping reveals that the Santoy shear zone, and deposits therein, show a clear structural relationship with the Carruthers Lake synform. The Santoy 7, Santoy “Gap”, and Santoy 8 deposits lie along the western limb of this structure while Santoy 8 East has been re-oriented by north-trending, 45°-plunging, S folds. The development of a splay off the D3 Tabbernor fault, located approximately 8 km east of the deposits, influenced the localization of strain along the western limb of the Carruthers Lake synform. This strain localization, in tandem with elevated fluid pressures, emplaced auriferous fault-fill quartz veins along ore zone parallel dykes during D3 dextral-reverse oblique slip movement. The age of the Lizard Lake pluton (1874.6 ± 2.9 Ma) and the ore zone adjacent hanging wall dyke (1874.6 ± 1.9 Ma) serves as a maximum mineralization age for these deposits. Structural relationships suggest that the Packman Lake pluton acted as a buttress during D3 deformation and produced a compressional jog in the Santoy shear zone that may have increased fracture density and fluid flow during mineralization. The age of this intrusion (1807 ± 7 Ma) serves as a maximum age for the development of this flexure. Gold mineralization at Santoy is closely associated with a calc-silicate alteration assemblage. This assemblage formed synchronously with the main stage mineralization event. Titanite and zircon within this assemblage was dated to 1755.5 ± 8.4 Ma (titanite) and 1754.0 ± 1.8 Ma (zircon) indicating syn-D3 deposit formation. A beryliferous pegmatite dyke that displays an F3 axial planar cleavage and crosscuts the auriferous ore lens at Santoy 8A has an age of 1736.1 ± 1.9 Ma. This age represents a minimum mineralization age for this system and a minimum age for regional D3 deformation in the Glennie domain.