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dc.contributor.advisorStavrinides, John
dc.contributor.authorSorout, Naveen
dc.date.accessioned2019-11-21T17:48:23Z
dc.date.available2019-11-21T17:48:23Z
dc.date.issued2019-07
dc.identifier.urihttp://hdl.handle.net/10294/9031
dc.descriptionA Thesis Submitted to the Faculty of Graduate Studies and Research In Partial Fulfillment of the Requirements for the Degree of Master of Science in Biology, University of Regina. vi, 68 p.en_US
dc.description.abstractThe rise of multi-drug resistant isolates of several pathogens, including Gram-negative members of the Enterobacteriaceae, requires the identification of new antimicrobials that can target them. Members of the genus Pantoea, a Gram-negative bacterial group, produce natural products with antimicrobial properties. A survey of a Pantoea collection against clinically relevant pathogens, including VRE, Enterobacter, Klebsiella, Citrobacter and Kosakonia, revealed 25 strains that produce at least one antibiotic against one or more of these pathogens. Of these strains, Pantoea agglomerans B025670 produces at least one antibiotic (denoted PNP-4) that has activity against strains of Enterobacter and Kosakonia. The conventional genetic approach was used to identify the antibiotic-producing biosynthetic gene cluster. This involved transposon mutagenesis of wild type B025670 (B025670-WT) followed by genetic screens on the mutants to identify candidates that no longer produced the antibiotic. The antibiotic production in B025670-WT was disrupted by mutations in several amino acid biosynthetic pathways, including mutations in histidine, leucine, arginine, isoleucine, cysteine and tryptophan operons. However, this was reversed upon supplementation with the corresponding amino acids in the growth medium. Furthermore, none of the candidate mutants appeared to have disruptions in the cluster responsible for PNP-4 production. Comparative genomics was then used to identify the mystery cluster. A comparison of B025670-WT draft genome with closely related non-producers of the antibiotic revealed two clusters that are unique to B025670-WT. Mutations in one of these clusters, potentially involved in fatty acid biosynthesis, results in a disruption of antibiotic production. Furthermore,the distribution of this cluster across Pantoea strains is limited.en_US
dc.language.isoenen_US
dc.publisherFaculty of Graduate Studies and Research, University of Reginaen_US
dc.titleIdentification of an antibiotic biosynthetic gene cluster in pantoea agglomerans B025670en_US
dc.typeThesisen
dc.description.authorstatusStudenten
dc.description.peerreviewyesen
thesis.degree.nameMaster of Science (MSc)en_US
thesis.degree.levelMaster'sen
thesis.degree.disciplineBiologyen_US
thesis.degree.grantorUniversity of Reginaen
thesis.degree.departmentDepartment of Biologyen_US
dc.contributor.committeememberHansmeier, Nicole
dc.contributor.committeememberDietz, Heather
dc.contributor.externalexaminerKumar, Ayush
dc.identifier.tcnumberTC-SRU-9031
dc.identifier.thesisurlhttps://ourspace.uregina.ca/bitstream/handle/10294/9031/Sorout_Naveen_MSC_BIOL_Fall2019.pdf


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