Targeted knockout of PpASCL, an anther-specific chalcone synthase-like gene, supports role in sporopollenin biosynthesis in the moss Physcomitrella patens.

dc.contributor.advisorSuh, Dae-Yeon
dc.contributor.authorDaku, Rhys Michael
dc.contributor.committeememberAshton, Neil
dc.contributor.committeememberDahms, Tanya
dc.contributor.externalexaminerWeger, Harold
dc.date.accessioned2015-07-22T19:20:13Z
dc.date.available2015-07-22T19:20:13Z
dc.date.issued2014-09
dc.descriptionA Thesis Submitted to the Faculty of Graduate Studies and Research In Partial Fulfillment of the Requirements for the Degree of Master of Science in Biochemistry, University of Regina. x, 69 p.en_US
dc.description.abstractThe anther-specific chalcone synthase-like (ASCL) gene of Physcomitrella patens, PpASCL, has been implicated in the biosynthesis of sporopollenin, the main constituent of exine and perine, the two outermost layers of the moss spore cell wall. Targeted knockouts of PpASCL were produced and the resulting mutants characterized. Knockout plants developed normally until late in sporophytic development, when the spores produced were aberrant and non-viable. The development of the spore cell wall appeared to be arrested early in microspore development, resulting in small, collapsed spores with irregular surface morphology. These results suggest that PpASCL plays an important role in the proper formation of the exine layer, and is required for the maturation and viability of moss spores.en_US
dc.description.authorstatusStudenten
dc.description.peerreviewyesen
dc.identifier.tcnumberTC-SRU-5850
dc.identifier.thesisurlhttp://ourspace.uregina.ca/bitstream/handle/10294/5850/Daku_Rhys_200201730_MSC_BIOC_Spring2015.pdf
dc.identifier.urihttps://hdl.handle.net/10294/5850
dc.language.isoenen_US
dc.publisherFaculty of Graduate Studies and Research, University of Reginaen_US
dc.titleTargeted knockout of PpASCL, an anther-specific chalcone synthase-like gene, supports role in sporopollenin biosynthesis in the moss Physcomitrella patens.en_US
dc.typeThesisen
thesis.degree.departmentDepartment of Chemistry and Biochemistryen_US
thesis.degree.disciplineBiochemistryen_US
thesis.degree.grantorUniversity of Reginaen
thesis.degree.levelMaster'sen
thesis.degree.nameMaster of Science (MSc)en_US
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