Distribution and Functional Analysis of an ATP-Binding Cassette Transporter-2 in Rhizobium legumionsarum

dc.contributor.advisorYost, Christopher
dc.contributor.authorAryal, Bijaya
dc.contributor.committeememberStavrinides, John
dc.contributor.externalexaminerNoel, Tanya C.
dc.date.accessioned2013-10-31T14:20:40Z
dc.date.available2013-10-31T14:20:40Z
dc.date.issued2012-12
dc.descriptionA Thesis Submitted to the Faculty of Graduate Studies and Research In Partial Fulfillment of the Requirements for the Degree of Master of Science in Biology, University of Regina., xii, 99 l.en_US
dc.description.abstractRhizobia are Gram negative, motile, non-sporulating soil bacteria, which interact with legume plants symbiotically to form nitrogen-fixing nodules. In the free-living state, rhizobia dwell in the rhizosphere and surrounding soil where they encounter various environmental stresses such as desiccation stress, nutrient and oxygen limitations, as well as temperature and pH fluctuations. To overcome such conditions, rhizobia adopt different survival mechanisms such as exopolysaccharide (EPS) production, swarming motility and biofilm formation. A previous study identified an ATP binding cassette transporter-2 (ABC) operon, which contains three genes, RL2975-RL2977 (here after referred as RL2975 transporter) that is involved in desiccation tolerance. However, the exact function of the transporter is not known. The study also reported that the transporter genes were present only in the R. leguminosarum and R. etli genomes sequences currently available in publicly accessible databases. Using bioinformatics, PCR and gene expression experiments, I examined the functional activities and diversity of the RL2975 transporter in Rhizobium species. PCR and subsequent DNA sequencing of the amplicons revealed that the transporter is predominantly constrained strains of R. leguminosarum but may also be found in some R. gallicum-like strains. Based on comparison between mutant and wild type, the transporter was not required for persistence in soil and swarming motility.en_US
dc.description.authorstatusStudenten
dc.description.peerreviewyesen
dc.identifier.tcnumberTC-SRU-3813
dc.identifier.thesisurlhttp://ourspace.uregina.ca/bitstream/handle/10294/3813/Aryal_Bijaya_200286944_MSC_BIOL_Spring2013.pdf
dc.identifier.urihttps://hdl.handle.net/10294/3813
dc.language.isoenen_US
dc.publisherFaculty of Graduate Studies and Research, University of Reginaen_US
dc.subject.lcshRhizobium leguminosarum
dc.subject.lcshATP-binding cassette transporters
dc.titleDistribution and Functional Analysis of an ATP-Binding Cassette Transporter-2 in Rhizobium legumionsarumen_US
dc.typeThesisen
thesis.degree.departmentDepartment of Biologyen_US
thesis.degree.disciplineBiologyen_US
thesis.degree.grantorUniversity of Reginaen
thesis.degree.levelMaster'sen
thesis.degree.nameMaster of Science (MSc)en_US
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